Rosetta Stone for HLA Haplotyping

Labcorp: ACC 20001604256 HLA DRB, DQ Typing Labcorp Test #;  167120 

 

DRB1

DQ

DRB3

DRB4

DRB5

Clinical

Multisusceptible

4

3

 

53

 

Severe RA, Malaria, Autoimmune hepatitis 3%

 

11/12

3

52B

 

 

Vaccine, athletes tall/hypermobile 1%

 

14

5

53B

 

 

0.1% incidence

Mold

7

2/3

 

53

 

 

 

13

6

52A,B,C

 

 

0.05% incidence

 

17

2

52A

 

 

 

 

18*

4

52A

 

 

 

 

 

 

 

 

 

 

Borrelia, post Lyme Syndrome

15

6

 

 

51

 

 

16

5

 

 

51

 

Dinoflagellates

4

7/8

 

53

 

 

Multiple Antibiotic Resistant Staph epidermidis (MARCoNS)

11

7

52B

 

 

 

Low MSH

1

5

 

 

 

 

No recognized significance

8

3,4,6

 

53

 

 

Low-risk Mold

7

9

 

53

 

 

 

12

7

52B

 

 

 

 

9

9

 

53

 

 

 

 

Rosetta Stone Interpretation:

1. Look at the LabCorp report. There are five categories of line entries: DRB1, DQ, DRB3, DRB4, and DRB5. Each individual will have two sets of three alleles, unless the DRB1 is 1, 8 or 10. Those patients will only have a DQ and won't have DRB 3, 4, 5. Each individual with a DRB1 other than 1, 8 or 10 will have a DQ and one other allele from DRB 3, 4, 5. If you are expecting to find two entries in, say DR 3, 4, 5 but only find one, the patient is homozygous for that allele and only one allele will appear on the PCR. Don't be worried that the numbers and letters look impossible to understand. They just have to be translated.

2. You will translate these categories into Bl, DQ, 52 (A, B, C), 53 and 51 respectively. If the translation were easy and made sense, you wouldn't need this

3. The numbers and letters in each of the 5 categories are given to an excessive amount of detail. Write down only the first two numbers in each line.

4. When you see 03 as one of the two genes, an allele, for DRB1, rewrite it as 17.

5. Record the entries in B3 by converting the 01 to A, the 02 to B and the 03 to C; this will give you 52A, 52B and 52C, respectively:

6. B4 is 53.

7. B5 is 51.

8. Record the genotypes in two columns, one each representing each parent, using the templates in the appendix.

9. When the international Language on HLA DR changes again, we might have to change the Rosetta Stone again. I hope not.

Wikipedia information on HLA;

en.wikipedia.org/wiki/History_and_naming_of_human_leukocyte_antigens

 

LabCorp testing;

HLA Testing Menu;

https://www.labcorp.com/wps/portal/!ut/p/c1/DcJbD0MwFADgX7Sc06LqcaTMQl2K4EVkRMzGLuKhv37L90ELf2t_zFO_z9vaP6CGlnUZZzKjkiKPqY_UYC5aniAYMJCX7TlCA63dlcJUJHQMTAJFMVQxUnY1KitDKKBGs1N3_CSaE6nFkeubLqLS3KOvHWsnSSNveRdDnQ5VXrpn9zQ0I4fXEkw_jwVIaw!!/

 

HLA A,B,C,DR1,3,4,5, DQ Intermediate Resolution

Test Number: 167096 CPT Code: 81370

Use; HLA antigens may be correlated with certain disease states or other clinical conditions. Indicate in clinical information which antigen(s) is of interest. Also used for screening of transplant candidates and potential donors, transfusion of specifically compatible blood products, among others.

Methodology; Sequence-based typing (SBT), sequence-specific oligonucleotide probes (SSOP), and/or sequence-specific primers (SSP) as needed to obtain the required resolution.

https://www.labcorp.com/wps/portal/!ut/p/c1/hYzJDoIwFEW_xQ8gfS1Q6hIUEQNFxlA2hAgxqIADwaRfL-4dcpfn3IMKNK-vpvZYje3QVxeUo4KWIaM8JJwA88kGiEot0Fc2BofOXHznAH_efDt0DRKoMMrU1mLsLlUInJiAG_tA6E7N9BBQgnLQyvgE90AyzKU9RfIgEy_VRu9h-HIZ7L3V-ZbU-b7OotQyLaUWDfvcVQONE04pe3fFepbET-na5U_MsBKai8ULp3E_lg!!/dl2/d1/L0lJWXBwZyEhL3dIRUJGUUJnc2FBTUJyQ0svWUk1eWx3ISEvN19VRTRTMUk5MzBPR1MyMElTM080TjJONjY4MC92aWV3VGVzdA!!/?testId=4076305

 

HLA DR panel;

https://www.labcorp.com/wps/portal/!ut/p/c1/hYzJDoIwFEW_xQ8wfW2h1CUQRAwUyhTKhhAhxgHnYNKvF_cOuctz7kE1mnZqx922fezOp_aIKlSzRnImJBEEeESWQChzwHQ9DD6buPrOAf68xeo89Eih2moKz8hwsKAQ-xmBIIuAsDUtTQkoRxUYTbaHW6w5FtobU73ReVgYj_BuRXoRJ6F7uOZdlXRlWji2M-9Uzz93aWwIIhjj765yJ0n9lC5D9cQcz6U9m70AH7UyMg!!/dl2/d1/L0lJWmltbUEhL3dQRUJGUUJoTlFCaERhQU1BWEtHL1lJNXlsdyEhLzdfVUU0UzFJOTMwT0dTMjBJUzNPNE4yTjY2ODAvdmlld1Rlc3Q!/?testId=4072122

 

 

Class II Antibody Panel;

Limitations; Detects antibodies of the IgG isotype and not IgM antibodies, which are usually indicative of autoantibodies.

https://www.labcorp.com/wps/portal/!ut/p/c1/hYzLCoJAFEC_KO511HFcapgZOr5ldCOSElZqDzGYr8_2RZzl4RyoYGVslv7UzP00NlcQUNE6ZpTHhBNkAdkhUamN-tZR0KWrL397xD81309DByVURp07Wqp4poqhmxL00gAJPaiFHiNkIFCr0zM-QskULp0lkUeZ-bk2-08jkGYY-dvLPWtF1BZJblv2pi079v2rhhonnFL2-ZY23AbxUhhab9N8SBQ!/dl2/d1/L0lDU0dZcHBnL0lKU0FDSXdrREFnaVFNQ0tZa0RBQ3VoZzRBISEvWUE0NXlsdyEvN19VRTRTMUk5MzBPR1MyMElTM080TjJONjY4MC92aWV3VGVzdA!!/?testId=3516802